|
|
|
|
Gap junction inhibitors 18-alfa-Glycyrrhetinic acid.
FW=470.7g/mol Either can be used, the beta form is more soluble. 1) Make a stock solution of 50mM in fresh DMSO (23.5mg in 0.5ml). DMSO must be fresh!!!!!. Do not use bottles that have been opened and closed several times. Use new ampulles, sold in aliquots of a few milliliters by Sigma (D2650) and discard unused DMSO after a week. 2) Dissolve to a final concentration of 25-100 mM in saline. Ref: GARY S. GOLDBERG,* ,1 ALONSO P. MORENO,† JOHN F. BECHBERGER,* STEPHEN S. HEARN,‡ RICHARD R. SHIVERS,§ DANIEL J. MACPHEE,§ YU-CHUN ZHANG,* AND CHRISTIAN C. G. NAUS* Evidence That Disruption of Connexon Particle Arrangements in Gap Junction Plaques Is Associated with Inhibition of Gap Junctional Communication by a Glycyrrhetinic Acid Derivative. EXPERIMENTAL CELL RESEARCH 222, 48–53 (1996) Main reference from Juan Carlos Sáez (jsaez@bio.puc.cl)
(±)-2-Octanol. FW = 130.2g/mol (It comes as a liquid) 1) First step is to dilute it to 5% in pure ethanol (100% EtOH). The density of octanol is 0.8g/ml. Thus, there are 0.4 g in 10ml of a 5% Octanol solution. 2) Dilute this solution to a final concentration in saline of 0.5-1.0mM. WARNING: in the STG, this blocker doesn't seem to work.
|
|
|
