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Cancer borealis Mn++ Saline:
|
Salt (Mol. weight, g/mol) |
Conc (mM) |
g/1lt |
g/2 lts |
g/5lt |
| KCl (74.56) |
11.0 |
0.82 |
1.64 |
4.10 |
| NaCl (58.44) |
440.0 |
25.72 |
51.43 |
128.57 |
| CaCl2.2H2O (147.02)
|
0.1 |
0.015 |
0.029 |
0.073 |
| MgCl2.6H2O (203.31)
|
26.0 |
5.288 |
10.575 |
26.438 |
| Trizma base (121.14) |
11.2 |
1.358 |
2.715 |
6.788 |
| Maleic acid |
5.1 |
0.593 |
1.185 |
2.963 |
| MnCl2.4H2O
(197.9 g/mol) |
12.9 mM |
2.55 |
5.10 |
10.20 |
pH 7.4-7.5
Mix and dissolve completely, then pH, and ONLY THEN
add the Mn++:
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