0.1M Phosphate buffer solution

If you don't remember how to prepare and dilute solution go here

Preparation:

- Prepare 4 volumes of 0.1 M sodium phosphate dibasic (Na₂HPO₄), i.e. 800ml for 1 lt solution
- Prepare 1 volume of 0.1 M sodium phosphate monobasic (NaH₂PO₄), i.e. 200 ml
- Mix 4 parts of the dibasic solution with 1 part of monobasic to make a final 0.1M phosphate solution.  Adjust the pH to 7.3-7.4

0.1M Phosphate buffer solution + 0.3% Triton-X

- Use the phosphate buffer made as indicated above and add Triton-X to a final concentration of 0.3% (i.e. 0.3 ml in 100 ml solution)

Careful: Triton-X is a very strong detergent. So, be careful because it will disolve skin !

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Last Modified:March 06, 2009