|
Cancer borealis
Saline:
Salt
(FW) |
Conc
(mM) |
g/lt |
g/6
lts |
g/11
lts |
KCl
(74.56) |
11.0 |
0.82 |
4.92 |
9.02 |
NaCl
(58.44) |
440.0 |
25.71 |
154.28 |
282.85 |
CaCl2.2H2O
(147.02) |
13.0 |
1.91 |
11.47 |
21.02 |
MgCl2.6H2O
(203.31) |
26.0 |
5.29 |
31.72 |
58.15 |
Trizma
base (121.14) |
11.2 |
1.36 |
8.14 |
14.92 |
Maleic
acid (116.07) |
5.1 |
0.59 |
3.55 |
6.51 |
Hepes
(instead of Tris+Maleate) (283.3) |
10.0 |
2.83 |
17.00 |
31.16 |
Supplement solution (to mix 50:50 with L-15 culture
medium):
Salt
(FW) |
Conc
(mM) |
g / lt |
g /
½lt |
g /
¼lt |
KCl
(74.56) |
16.40 |
1.22 |
0.61 |
0.305 |
NaCl
(58.44) |
743.66 |
43.46 |
21.73 |
10.865 |
CaCl2.2H2O
(147.02) |
24.74 |
3.64 |
1.82 |
0.909 |
MgCl2.6H20
(203.31) |
50.20 |
10.21 |
5.10 |
2.552 |
Hepes
(283.3) |
10.0 |
2.83 |
1.42 |
0.708 |
Zero Ca++ / zero Mg++
dissociation solution
Salt
(FW) |
Conc
(mM) |
g / lt |
g /
½lt |
g /
¼lt |
KCl
(74.56) |
11.0 |
0.82 |
0.41 |
0.205 |
NaCl
(58.44) |
440.0 |
25.71 |
12.86 |
6.43 |
Hepes
(283.3) |
10.0 |
2.83 |
1.42 |
0.708 |
pH = 7.4-7.5
Osmolarity = 1030 mOsm
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