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Procambarus clarkii
Saline:
Salt |
Conc (mM) |
g/lt |
g/2lt |
g/6lt |
KCl (74.56) |
5.4 |
0.403 |
0.805 |
2.416 |
NaCl (58.44) |
195.0 |
11.396 |
22.792 |
68.375 |
CaCl2.2H2O
(147.02) |
13.5 |
1.985 |
3.970 |
11.909 |
MgCl2.6H2O
(203.31) |
2.6 |
0.529 |
1.057 |
3.172 |
Trizma base (121.14) |
11.2 |
1.357 |
2.713 |
8.141 |
Maleic acid (116.07) |
5.1 |
0.592 |
1.184 |
3.552 |
Hepes (instead of
Tris+Maleate) (283.3) |
10.0 |
2.833 |
5.666 |
16.998 |
pH = 7.4-7.5
Supplement solution (to mix 50-50 with Leibovitz L15 culture
medium):
Salt |
Conc (mM) |
g / lt |
g / ½lt |
g / ¼lt |
KCl (74.56) |
4.64 |
0.346 |
0.173 |
0.086 |
NaCl (58.44) |
253.10 |
14.791 |
7.396 |
3.698 |
CaCl2.2H2O
(147.02) |
24.74 |
3.637 |
1.819 |
0.909 |
MgCl2.6H20
(203.31) |
4.22 |
0.858 |
0.429 |
0.214 |
Hepes (283.3) |
10.0 |
2.833 |
1.417 |
0.709 |
pH = 7.4 - 7.5
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